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Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

 Website: https://lolina.dk

Product Specification

Product Description

Lolina? Mesenchymal  Stem  Cell  Chondrogenic  Differentiation   Supplement  (MCDS)  is  a  medium supplement  designed  for  differentiation  of  primary  mesenchymal  stem  cells  (MSCs)  to  an chondrogenic lineage as determined by Safranin-O staining in vitro. It is a sterile, concentrated (100X)  solution.  The  supplement  is  designed  as  an  additive  for  mesenchymal   stem  cell chondrogenic  differentiation  medium  (MCDM)  and   should  be  used  in conjunction with that medium.

Prepare for use

Thaw MCDS at 37^oC. Gently tilt the MCDS tube  several times during thawing to help the contents  dissolve.  Make  sure  the  contents  of the  supplement  are  completely  dissolved  into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add MCDS, P/S solution and TGF-β3, which is provided by the customer, into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several  components  of  differentiation  medium  are  light-labile,  it  is  recommended  that  the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37^oC. When stored in the dark at 4^oC, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

Note

MCDS is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

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